The goal of the Cellular and Molecular Radiobiology Laboratory is to develop and field biodosimetry capabilities for the assessment of acute, protracted, and prior radiation exposures. This work is based on a fundamental understanding of molecular mechanisms inherent in the induction and repair of radiation injury that are expressed as acute and late effects. The aim is to develop cytological, molecular, and hematological biomarkers that are suitable for high throughput and rapid analysis. The studies typically use human tissues/cell samples, such as peripheral blood lymphocytes, animal tissues/cells/plasma from in vivo radiation models, and human radiation accident data or samples. Cytological studies involve the measurement of radiation-induced DNA and chromosome injury, using the premature chromosome condensation, dicentric, and translocation assays. Other biomarker endpoints involve the use of molecular biology techniques (enzyme linked immunosorbent assays, fluorogenic polymerase chain reaction assays) and advanced technologies (deployable blood cell counters and multiple target antibody based analysis systems). Our strategy is to identify, validate, and optimize an integrated analysis systems using multiparameter assays including alternative molecular biomarkers (gene expression, protein biomarkers, and DNA mutations) for the measurement of prompt and persistent radiation injury endpoints. In addition, research efforts also focus on the development of tools to facilitate the collection of biodosimetry data supporting radiation casualty management and risk communication.

 

Keywords:

Radiobiology; Molecular biology; Cell biology; DNA; Genetic markers; Dosimetry; Physiological effects of radiation; Mutation; DNA probes;

Citizenship:  Open to U.S. citizens

Level:  Open to Postdoctoral and Senior applicants