NRC Research and Fellowship Programs
Fellowships Office
Policy and Global Affairs

Participating Agencies

RAP opportunity at National Institute of Standards and Technology     NIST

Advanced Imaging Tools to Measure Dynamics of Pluripotency and Differentiation


Material Measurement Laboratory, Biosystems and Biomaterials Division

opportunity location
50.64.41.B8384 Gaithersburg, MD

NIST only participates in the February and August reviews.


name email phone
John Timothy Elliott 301.975.8551
Michael Halter 301.461.0956
Alexander W Peterson 301.975.5665
Anne L. Plant 301.975.3124


Induced pluripotent stem cell technologies are powerful new tools for biomedical research and have the potential to revolutionize medicine. The mechanisms by which cells transition from pluripotent to differentiated states is incompletely understood, and correlating measurable parameters to identify efficient culture conditions and release criteria for safe and effective therapies is imperfect. One challenge is the natural biological variability in cell responses across a population. In order to provide better biomarkers  of pluripotency and differentiation, data describing the changes in gene expression at the single cell level are needed. In this project, quantitative live cell imaging and image analysis will be used to follow gene expression dynamics, and other phenotypic characteristics, in single cells. Imaging modes that employ fluorescence, transmitted light, quantitative phase, and/or surface plasmon resonance microscopy may be used to acquire different kinds of images on large numbers of iPS cells in culture; machine learning algorithms and other image analysis strategies may be used to extract and test image features as predictors of cell stateQuantitiation of the extent, probability, and dynamics of changes in phenotypic markers over the population will add confidence in the interpretation of biomarkers of pluripotency and differentiation.


Bhadriraju K, et al: “Large-scale time-lapse microscopy of Oct4 expression in human embryonic stem cell colonies.” Stem Cell Research (17): 122-129, 2016

Peterson, Alexander W.; Halter, Michael W.; Tona, Alessandro; Plant, Anne L. (2014) High Resolution Surface Plasmon Resonance Imaging of Cells. BMC Cell Biology. 15:35 DOI: 10.1186/1471-2121-15-35

Peterson, Alexander, Michael Halter, Anne Plant, and John Elliott (2016) Surface plasmon resonance microscopy: achieving a quantitative optical response. Review of Scientific Instruments, 87:9 DOI: 10.1063/1.4962034

key words
Bioengineering; Quantitative imaging; Stem cell; Pluripotency; Differentiation; Live cell microscopy; Image analysis; Biological noise; Machine learning;


Citizenship:  Open to U.S. citizens
Level:  Open to Postdoctoral applicants


Base Stipend Travel Allotment Supplementation
$82,764.00 $3,000.00
Copyright © 2024. National Academy of Sciences. All rights reserved.Terms of Use and Privacy Policy