Opportunity at National Institute of Standards and Technology NIST
Engineering Enzymes for the Biomanufacture of Nucleoside Conjugates
Material Measurement Laboratory, Biomolecular Measurement Division
Please note: This Agency only participates in the February and August reviews.
Nucleoside analogues are widely used as antiviral and antitumor drugs by blocking the activity of DNA or RNA polymerases. An archaeal enzyme that converts a nucleotide triphosphate (NTP) to a nucleotide monophosphate (NMP)-small molecule (SM) adduct via formation of a NMP-O-R or a NMP-N-R linkage was recently identified in the laboratory. While the biological significance of this activity is unknown, its utility as a potentially inexpensive and bio-friendly method for biomanufacturing nucleoside analog “pro-drugs” is being explored. Ongoing research focuses on determining the efficiency and selectivity of the enzyme reaction by exploring the chemical space of SMs (i.e., sugars, lipids, amino acids) as well as nucleoside analogues (i.e., ddNTPs) that can used by the enzyme as substrates to generate NMP-SM.
Galal WC, Pan M, Kelman Z, Hurwitz J: Journal of Biological Chemistry 287: 16209, 2012
Galal WC, et al: Journal of Biological Chemistry 287: 16220, 2012
Antiviral drugs; Anticancer drugs; Nucleoside analogues; Primase;
Open to U.S. citizens
Open to Postdoctoral applicants