In the field of tissue engineering, cells and scaffolds are often combined to yield constructs that are used as therapeutics to repair or restore tissue function in patients. Often viable cells are required to achieve the intended mechanism of action for the therapeutic, where the live cells may build new tissue or may release factors that induce tissue regeneration. Thus, there is a need to be able to reliably measure cell viability in scaffolds as a quality attribute of a tissue engineered medical product.  We will develop new methods for assessing cell viability in scaffolds that have advantages such as being label-free, non-destructive, multiparametric, validated or more accurate.