This research aims to advance infrared (IR) and Raman imaging techniques for determining the molecular composition and orientation of biomaterials and other complex new materials, e.g., 3D printing and shape memory polymers. We have demonstrated that rapid hyperspectral imaging based on IR and coherent Raman can unravel the complex structure of polymers, including 3D orientation angles. This research aims to develop the label-free chemical imaging method to investigate the microscopic structure and composition of new biomaterials and smart polymers via collaboration with material scientists and biomedical engineers biologists.